Instructions

Guidelines for Abstract submission

First page:

Title and authors

Up to 4 Keywords

Choose an appropriate theme

Indicate if submission for a poster or oral presentation 

Second page:

Title: Title of the abstract should highlight the main findings. Do not exceed 200 characters (about 20 words).

Style: Times font, size 12 and written in capital and bold letters.

Authors: Authors’ names must be separated by commas: Surname, abbreviation name (1), Surname, abbreviation name (2), Surname, abbreviation name (3) (e.g. Smith, J.(1), Gonzales, R.(2), Dupont D. (3)). The speaker name should be underlined (Smith J.(1)).

Style: Times font, size 12 and written in small letters.

Authors affiliations: In case of multiple affiliations, use superscript number in parentheses in the list of authors (i.e. Smith, J. (1), Gonzales, R. (2) …) and refer to this number (1)Department of anthropology, Washington University, USA. Style: Times font, size 12.

Abstract: The abstract should not exceed 2800 characters (about 400 words) and must be subdivided in the following subsections: Introduction, Methods, Results and Discussion, Conclusion. “Introduction” should briefly outline the context and purpose of the study; “Methods” describe the main methods used to conduct the study; “Results and Discussion”, summarizes and discusses the main findings; and “Conclusion” gives a short summary and the potential implications resulting from this work. Gene abbreviations must be defined when first mentioned. Do not cite references in the abstract. Do not include figures or tables.

Style: Subsections titles must be written in bold and small letters. Times font, size 12.

File format and name:

The paper format is A4 (11.69in/29.7cm height and 8.27in/21cm width). Left and right

margins are 1 inch (2.5 cm). The abstract should be formatted as the sample abstract below.

The easiest way to format your abstract is to paste over your unformatted text in the

corresponding section of the template provided in the Abstract Submission Form. Use the “paste special, unformatted text” option of your word processing software.

The file of the Abstract Submission Form should be saved in Rich Text Format (RTF) as

Doe_John.rtf. Add increasing number in case of multiple submissions per submitter as follow

Doe_John1.rtf. Upload the abstract at http://ccmar.ualg.pt/10ISRPF/abstracts

The submission deadline is February 12th, 2018.

Page 1

GONADAL SOMA-DERIVED GROWTH FACTOR SUPPRESSES THE EXPRESSION OF FOLLICLE-STIMULATING HORMONE RECEPTOR IN MEDAKA.

Uchikawa, T.(1), Kobira, H.(1), Hirai, T. (2), Kitano, T.(1)

(1)Graduate School of Science and Technology, Kumamoto University, Kumamoto 860-8555, Japan. (2)Depertment of Biosciences/Biotechnology Research Center, Teikyo University of Science and Technology, Yamanashi 409-0193, Japan. email: 114d9009@st.kumamoto-u.ac.jp

Keywords: medaka, follicle-stimulating hormone receptor, gonadal soma-derived growth factor

The authors would like to communicate this work as (delete the less preferred)

• oral presentation

Choose one theme based on the main subjects of the Sessions (e.g. Sex determination, Sex differentiation, Stem cells and spermatogenesis, etc).

Sex determination

Page 2

GONADAL SOMA-DERIVED GROWTH FACTOR SUPPRESSES THE EXPRESSION OF FOLLICLE-STIMULATING HORMONE RECEPTOR IN MEDAKA.

Uchikawa, T.(1), Kobira, H.(1), Hirai, T. (2), Kitano, T.(1)

(1)Graduate School of Science and Technology, Kumamoto University, Kumamoto 860-8555, Japan. (2)Depertment of Biosciences/Biotechnology Research Center, Teikyo University of Science and Technology, Yamanashi 409-0193, Japan. email: 114d9009@st.kumamoto-u.ac.jp

Introduction

Medaka (Oryzias latipes) is a teleost fish that has a XX/XY sex determination system. We previously showed that the expression levels of follicle-stimulating hormone receptor (fshr) mRNA in XX medaka were higher than those in XY fishes at the hatching stage. Moreover, cortisol caused masculinization as well as suppression of fshr expression in XX embryos, suggesting that FSHR may be involved in the ovarian differentiation in medaka. However, the molecular mechanism of transcriptional regulation of fshr gene in medaka remains unclear. In this study, to analyze the effect of gonadal soma-derived growth factor (GSDF), a member of the TGFβ superfamily which is male-specifically expressed in the gonadal somatic cells, on fshr expression regulation in medaka, we established fshr-GFP transgenic (Tg) medaka and gsdf-overexpressed Tg medaka lines.

Methods

Fshr-GFP Tg medaka line was generated by injecting the EGFP vector fused to the regulatory region of the medaka fshr gene into fertilized eggs of the FLFII stock. All injected embryos were bred to adults and only F1 embryos possessing GFP fluorescence were selected and used to produce succeeding generations. Gsdf-overexpressed (mis-gsdf) Tg medaka line was generated by simultaneously injecting the mis-gsdf and olvas-DsRed vectors. F1 embryos possessing DsRed fluorescence were selected and used to produce succeeding generations. We then mated the fshr-GFP Tg medaka line with mis-gsdf Tg medaka line.

Results and Discussion

We first established fshr-GFP Tg medaka line and examined the expression patterns of GFP and FSHR in the Tg medaka by immunohistochemistry. The expression patterns of GFP were coincident with those of FSHR in Tg medaka, suggesting that GFP expression reflects FSHR expression in this line. On the other hand, we successfully generated the mis-gsdf Tg medaka line which expressesgsdf mRNA in the somatic cells of XX gonads, and the Tg XX fishes became typical males which are fertile. To investigate whether GSDF regulates fshr and GFP expression in fshr-GFP Tg medaka, we examined the expression pattern of fshr and GFP mRNAs in fshr-GFP/mis-gsdf Tg fishes by quantitative real-time PCR. As results, the expression levels of fshr and GFP mRNAs were significantly suppressed in the gonads of the Tg XX fishes.

Conclusion

In the present study, we showed that GSDF inhibited the expression of GFP and fshr mRNAs in fshr-GFP/mis-gsdf Tg medaka, suggesting that GSDF down-regulates the expression of fshr and induces male development in XY medaka.